Establishment of heterotopic ossification via sharp instrument injury in rats
نویسندگان
چکیده
OBJECTIVE To establish an animal model for heterotopic ossification (HO) induced by sharp instrument injury in Sprague-Dawley (SD) rat and to investigate its possible mechanism. MATERIALS AND METHODS A total of 48 male SD rats were divided into 3 groups (n=16). In sham group, incision and suture were performed only in the left leg. Partial tenotomy was performed in the left Achilles tendons in the PAT group. In Achilles' tenotomy (AT) group, tenotomy was performed in the left Achilles tendons to establish animal model of EO. X-ray and histological examinations were made at 6 and 10 weeks after operation. RESULTS No HO occurred in the sham and PAT groups. In AT group, X-ray results on 4 rats showed cartilage and bone formation while the remaining 4 rats showed chondrification in histological examination at 6 weeks after operation. At 10 weeks all rats showed bone formation with trabecular bone. This kind of HO usually develops through a process of endochondral ossification. CONCLUSION Tenotomy is a simple, effective and feasible method to induce HO.
منابع مشابه
Progression of Heterotopic Ossification around the Elbow after Trauma
Background: This study addresses the null hypothesis that there is no expansion of heterotopic ossification (HO) in the elbow beyond what can be seen early on. Methods: The area of HO was measured on lateral radiographs of 38 consecutive patients that had operative treatment of HO between 2000 and 2013. Measurements from radiographs obtained between 3 to 7 weeks were compared to measurements...
متن کاملتغییرات رادیوگرافیک ستون فقرات کمری و لگن در بیماران با ضایعه نخاعی مزمن
Background: Chronic spinal cord injury has an important role in sensorimotor disor-ders which is associated with many complications and the rate of these complications should be certainly considered. The most important complications are the changes in bones and joints which come with pain and lead to increase in their motor disability. In this paper, the radiographic images were studied and the...
متن کاملHeterotopic Ossification around the Knee after Internal Fixation of a Complex Tibial Plateau Fracture Combined with the Use of Demineralized Bone Matrix (DBM): A Case Report
Demineralized bone matrix has been successfully commercialized as an alternative bone graft material that not only can function as filler but also as an osteoinductive graft. Numerous studies have confirmed its beneficial use in clinical practice. Heterotopic ossification after internal fixation combined with the use of demineralized bone matrix has not been widely reported. In this paper we de...
متن کاملHeterotopic ossification following combat-related trauma.
Introduction The term heterotopic ossification refers to the aberrant formation of mature, lamellar bone in nonosseous tissue. Translated from its Greek (heteros and topos) and Latin (ossificatio) etymologic origins, heterotopic ossification can be literally defined as "bone formation in other location." The first written account of heterotopic ossification describes the treatment of symptomati...
متن کاملIncidence and risk factors of heterotopic ossification following major elbow trauma.
Heterotopic ossification is a common complication of Orthopaedic Trauma Association (OTA) type C distal humerus fractures and ulnohumeral fracture dislocations. The purpose of this study was to precisely define the incidence of heterotopic ossification following major elbow trauma and to identify risk factors for the development of clinically significant heterotopic ossification and for surgica...
متن کامل